Evaluation of novel diagnostic techniques for contagious bovine pleuropneumonia (CBPP) in Uganda

Abstract

Field surveys were conducted in Mbarara, Bugiri and Kamuli districts suspected to have CBPP in order to get blood samples and nasal swabs from infected and non-infected cattle. Cattle were examined clinically for CBPP. Blood samples were taken from the animals for Complement Fixation Test (CFT) and competitive ELISA (cELISA). Nasal swabs or tracheal scrapings from the same suspected cattle were mixed with PBS buffer for extraction of Mycoplasma mycoides DNA. A total of 954 blood samples were collected for preparation of serum samples for CFT and cELISA, and 174 nasal swabs for DNA preparation for subsequent use in PCR. A total of 621 out of 907 serum samples from Mbarara district have been analysed by CFT and cELISA (Tablel). Eleven of 907 serum samples were positive by CFT while 16 were positive by cELISA. The two tests had a concordance of 97.2%, which indicates good agreement in the results of the two tests evaluated.